Oxford Nanopore Duplex sequencing of HG002 - SRA

SRX23904595: Oxford Nanopore Duplex sequencing of HG002
1 OXFORD_NANOPORE (PromethION) run: 383,898 spots, 10.3G bases, 7.3Gb downloads

Design: ONT Duplex sequencing: High molecular weight DNA was extracted from cells using NEB Monarch HMW DNA Extraction Kit for Tissue (NEB T3060). Isolated DNA was then sheared using the Diagenode Megaruptor 3, DNAFluid+ Kit (E07020001). The size of sheared DNA fragments were analyzed on an Agilent Femto Pulse System using the Genomic DNA 165kb Kit (FP-1002-0275). The fragment size distribution of post-sheared DNA had a peak at approximately 50kbp. Small DNA fragments were removed from the sample using the PacBio SRE kit (SKU 102-208-300). Library preparation was carried out using Oxford Nanopore Technologies Ligation Sequencing Kit V14 (SQK-LSK114). PromethION high duplex flow cells were used for sequencing on PromethION 48 sequencer. Three libraries were prepared per flow cell. Flow cells were washed using ONTs Flow Cell Wash Kit (EXP-WSH004) and reloaded with fresh library every 24 hours for a total sequencing runtime of 72 hours.

Submitted by: UCSC Genomics Institute (UCSC GI)

Study: HPRC PLUS Genome Sequencing

PRJNA731524 • SRP320775 • All experiments • All runs

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This project contains genomic sequencing data used by the Human Pangenome Reference Consortium (HPRC) which was not produced using funds for the HPRC. Data types include, but are not limited to: PacBio HiFi, Oxford Nanopore, Hi-C/Omni-C. Data are used for assembly of genomes and pangenomes as well as assembly analysis and quality control. The Human Pangenome Reference Consortium aims to create a collection of high-quality reference genomes that represent the genetic diversity of the human population.

Sample: NIST HG002 NA24385

SAMN03283347 • SRS817069 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: 11_15_22_R1041_Duplex_HG002_1

Instrument: PromethION

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 383,898 spots, 10.3G bases, 7.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR28295771	383,898	10.3G	7.3Gb	2024-03-12

ID:: 32209619

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Sequence Read Archive

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